Comparative Evaluation of Whole Urine and Pellet-Based Extraction Workflows for Urine Liquid Biopsy
Explore a recovery-focused approach to processing whole urine without separating the specimen into independent cellular and cell-free workflows.
This white paper describes two complementary studies evaluating whole-urine single extraction versus fractionated processing using the nRichDX Revolution Plus Sample Prep System and Revolution cfTNA workflow.
Does urine need to be divided before the molecular workflow begins?
Urine contains molecular material across both cell-free and cell-associated compartments.
Traditional urine-processing workflows may separate the specimen into pellet and supernatant fractions before extraction. That approach introduces additional handling, processing steps, transfers, and ultimately separate molecular workflows.
The nRichDX white paper examines an alternative approach: processing whole urine directly in a single extraction so that molecular material from both compartments can enter one upstream sample-preparation workflow.
Fractionate the sample, or process the whole specimen.
The white paper evaluates these upstream approaches under comparable study conditions.
Fractionated Processing
Whole-Urine Single Extraction
Follow the specimen through the upstream workflow.
The paper focuses on how workflow architecture affects the molecular material carried forward from urine.
Whole urine contains more than the cell-free fraction.
Evaluate a workflow intended to retain molecular material associated with both the cellular and cell-free components of the specimen.
Reduce unnecessary fractionation and transfers.
Compare direct whole-urine processing with workflows that divide the original specimen before extraction.
Evaluate what reaches downstream analysis.
Examine nucleic-acid recovery as an important upstream consideration before downstream molecular testing begins.
Get the full study discussion.
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Download the 2026 Urine White Paper
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Explore the data behind the whole-urine workflow.
The related 2026 nRichDX poster presents the comparative whole-urine and fractionated-processing study and is available directly in the scientific resource library.