The Revolution Plus System
Smart, easy-to-use semi-automated extraction using nRichDX's Revolution kits for cfDNA, cfTNA/cfRNA, and CTCs
The Cartridge Makes the Difference
The unique nRicher Cartridge maximizes yield and purity by eliminating problems found in other methods. Only from nRichDX
Upcoming RNA LBx Webinar
Title: "Current Perspectives on RNA Liquid Biopsy: Impact of RNA Extraction on Assay Performance"
Date/Time: Thursday, October 16th at 9am PST/Noon EST/6pm CET
Investigating Cell-Free RNA?
nRichDX's Revolution cfTNA Max 20 and Max 50 Kits extract cell-free circulating Total Nucleic Acid, including cfRNA and ctRNA
JHU Lab Publication Features Revolution System
Full-Length Clonal Immunoglobulin Rearrangements in cfDNA: Improved Recovery and Sequencing by Xian, R. et. al
Dartmouth Lab Validates Revolution System
Standardized Workflow and Analytical Validation of Cell-Free DNA Extraction for Liquid Biopsy Using a Magnetic Bead-Based Cartridge System by Sathyanarayana, S. et al.
See you at AMP2025!
Exhibiting 13-15 November 2025
Booth #1029
Boston Convention & Exhibition Center
Not Sure Which Revolution Kit to Use?
See a summary table of Revolution Sample Prep Kits currently available on the Revolution Plus Sample Prep System.
The Revolution Sample Prep System
For your liquid biopsy requirements today... and tomorrow
Automation for the nRicher Cartridge
The nRichDX Revolution Plus
Extraction of up to 12 samples per run in a semi-automated workflow
The Simple Math
Analyte yield is a function of input sample volume and recovery rate
As sample volume increases, analyte yield increases. Note the increasing intensity of rarer species of cfDNA indicated by the dimer and trimer forms with increasing sample volume. Competing methods are often limited to 10 mL or less of input sample volume per extraction.
Probability of detection of rare alleles at a low VAF of 0.1% or even 0.01% depends on a sufficiently high cfDNA input into the detection assay. Optimal input is a simple function of sample volume and recovery rate. Competing sample prep systems are lacking in both sample volume capacity and recovery rate, and therefore don’t produce sufficient yield to ensure a high probability of allele detection. This is a leading cause of Quantity Not Sufficient (QNS) errors in liquid biopsy assays.
Why Other Sample Prep Methods are the Problem
They use yield-lowering procedures such as sample transfers, pre-extraction sample volume concentration, re-elution, and pooling steps
- Sample Transfers - moving the sample from plasticware to plasticware during the extraction protocol, leaving small amounts of material behind with each sample transfer - including targeted rare analytes
- Pre-extraction Sample Volume Concentration - Reducing the input sample volume to accommodate inherent volume limitations of the method
- Re-elution of the extraction eluate - attempting to increase yield of analyte by passing the eluted sample over the extraction matrix repeatedly to boost yield of rare analytes
- Pooling steps - extracting the same sample using multiple extractions and combining the eluates, which may require subsequent eluate volume concentration
Other methods, including automated methods, are inherently sample-volume limited and must use one or more of the above yield-lowering schemes to extract from volumes greater than 5 mL - 10 mL. These methods were not specifically engineered for extraction of rare analytes such as ctDNA and ctRNA which often leads to lower yields. Lower yields of analytes in the assay lowers assay sensitivity and limit of detection and may result in Quantity Not Sufficient (QNS) errors.
A Growing Range of Liquid Biopsy Sample Types and Applications
