Revolution CTC Enrichment Kit (Epithelial Origin)

Easily isolate intact CTCs displaying the EpCAM epitope from blood samples 

Small box with CTC label added2

Revolution CTC Enrichment Kit

PN 407000

Product Summary:

  • Gentle Processing- Preserves CTC viability and morphology; supports downstream cell culture
  • High Sensitivity - Efficiently enriches  CTCs from as little as 1 mL up to 50 mL in a single extraction (inquire for larger sample volumes of up to 100 mL per extraction)
  • Simplified Workflow - Streamlined protocol with fewer steps and shorter processing time

The nRichDX Revolution CTC Enrichment Kit (Epithelial Origin) uses proprietary magnetic bead technology and anti-EpCAM antibodies to isolate intact, viable epithelial-derived circulating tumor cells (CTCs) from whole blood.

Each kit includes reagents and 12 x 50 mL nRicher Cartridges, enabling high-sensitivity, label-dependent CTC isolation using the nRichDX Revolution System (sold separately). Reagents provided support up to 12 isolations from samples of up to 10 mL per cartridge. Additional reagents are available via the Revolution CTC Reagent Kit - Epithelial Origin (PN 407600).

For Research Use Only. Not for use in diagnostic procedures.

Resources:

REVOLUTION CTC REAGENT KIT (EPITHELIAL ORIGIN) forWeb

Revolution CTC Reagent Kit

PN 407600

The Revolution CTC Reagent Kit supplies supplemental reagents to enable complete utilization of any unused nRicher Cartridges contained in the Revolution CTC Enrichment Kit - Epithelial Origin (PN 407000 - purchased separately). The Revolution CTC Reagent Kit does not include nRicher Cartridges.

The kit contains the reagents necessary for label-dependent CTC enrichment using the nRichDX Revolution System (purchased separately). Reagent volumes in the Reagent Kit are the same as in the Revolution CTC Enrichment Kit - Epithelial Origin (PN 40700) and are adequate for qty. 12 CTC isolations of up to 10 mL of sample per nRicher Cartridge.

For Research Use Only. Not for use in diagnostic procedures.

Resources:

Performance Data

CTC Recovery

CTC % Recovery 2

Figure 1 - Percent Recovery of 100 MCF-7 cells spiked into 10 mL human whole blood using the Revolution CTC Enrichment Kit (Epithelial Origin) replicated in separate CTC enrichments over 3 days.

 

Immunofluorescent Microscopy Images (DAPI, EpCAM, CK8) Single Cells and Cell Clusters

Representative images of potential CTCs isolated using the nRichDX Revolution CTC Enrichment Kit (Epithelial Origin):

DAPI EpCAM CK8 images_Single Cells

Figure 2 - Differential protein expression of EpCAM (green), CK8 (orange) displayed by immunofluorescence staining of the isolated cells. DAPI (blue). 800x magnification.

 

Representative images of potential clusters of CTCs isolated using the nRichDX Revolution CTC Enrichment Kit (Epithelial Origin):

DAPI EpCAM CK8 images_Cell Clusters

Figure 3 - Differential protein expression of EpCAM (green), CK8 (orange) displayed by immunofluorescence staining of the isolated cells. DAPI (blue). 600x magnification.

Figures 2 and 3 images were taken using the Zeiss LSM 900 with Airyscan Confocal Microscope.

 

 

Get More Analytes and Information from Your Blood Sample

Together with other nRichDX Revolution Sample Prep Kits, The Revolution CTC Enrichment Kit (Epithelial Origin) may be used in tandem with other Revolution Kits to enrich CTCs, and isolate circulating cell-free total nucleic acid (cfTNA), cfDNA, and cfRNA from the same whole blood sample as shown in the figure below:

CTC Related Infographic for Website

Figure 3 - Overview of enrichment of CTCs, and extraction of cfDNA, and cfRNA using Revolution Kits.

For a description of enrichment of CTCs, and extraction of cfDNA, and cfRNA from the same blood sample using the Revolution System, please see the poster from Jafari et al. titled, "Extraction of Multiple Analytes in Liquid Biopsy May Improve the Diagnosis of Breast Cancer" presented at the Cancer Genomics Consortium 2023 Meeting. 

 

Revolution System Kits Work Together

TapeStation traces demonstrating integrity and yields of cfRNA extracted using the Revolution cfTNA Max 20 Kit from cells first isolated using the Revolution CTC Enrichment Kit (Epithelial Origin):

RNA extracted with Revolution cfTNA Kit from MCF7 cells Isolated with Revolution CTC Kit

 

Figure 4 - The Revolution cfTNA Kit was used to extract cfRNA from 103, 104 and 106 MCF-7 cells spiked into 10 ml of whole blood and captured using the Revolution CTC Enrichment Kit. 

 

*The Revolution CTC Enrichment Kit (Epithelial Origin) is not labeled for IVD-use, despite the fact that the Revolution cfDNA and cfTNA Kits and Revolution Sample Prep System hardware are IVD-labeled.

For Research Use Only. Not for use in diagnostic procedures.

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