What is liquid biopsy and how does nRichDX's Revolution Sample Prep System help? 

HubSpot Video

Insufficient liquid biopsy assay inputs can result in consequential false negatives

nRichDX delivers exponentially more target input

Circulating cell-free DNA (cfDNA) is extremely rare, especially in early stages of cancer detection. Many competing sample prep systems were originally designed for applications that had plentiful targets in small samples (100 μl - 5 mL), not for the enrichment of cfDNA from liquid biopsies.

These methods are unable to achieve sufficient yields to detect the low allele frequency rates required for liquid biopsy testing, making them ineffective as an early detection diagnostic tool.

nRichDX-yield-referenced

The simple math

Yield is a function of sample volume and recovery rate.

simple-math

Accuracy of allele detection depends on a sufficiently high cfDNA input, and optimal input is a simple function of sample volume and recovery rate. Competing sample prep systems are lacking in both volume and recovery rate, and therefore don’t produce sufficient yield to ensure a high probability of allele detection.

Volume matters more than recovery rate

High efficiency alone is not enough. A larger volume of starting material directly impacts the success of downstream tests, making it possible to detect very early stage cancers by liquid biopsy

In order to achieve a 99.99% chance of cfDNA detection at an allele frequency of 0.05%, you'll need at least 83ng of cfDNA in your assay†. Even with 100% efficiency, you won't be able to get 83ng out of 4ml of sample. To increase cfDNA yield, you’ll need to increase both sample volume and extraction efficiency

This can be accomplished using nRichDX’s high-efficiency Revolution System, which permits single-cartridge processing of sample volumes from 1 mL to 20 mL.

Competitors have neither the ability to process large sample volumes nor the extraction efficiency to achieve the high cfDNA yields needed to ensure reliable detection.

† Nature Communications Volume 8, Article number: 15086 (2017)

Yield-graph

The nRichDX Revolution Sample Prep System.

Larger volumes, greater efficiency, unprecedented yields, highly confident results.

Revolution System_12 Position Rack

Industry-leading yield

Higher yield illustration

nRichDX has the widest sample input range of any system, from 1 to 20 mL; several times more than from competing technologies (500 µl - 4 mL).

90%

recovery rate*

Compare to typical 40% - 50% recovery rate in competing systems.

* Customer evaluation study

1 mL — 20 mL

sample input volumes

Compare to typical sample volumes of 4 mL or less in competing systems.

>95%

results accuracy

Compare to typical 50% result accuracy in competing systems

Samples and Applications diagram_forweb-1

Ready to learn more?

Request demo

Keep up to date with nRichDX

You’ll be the first to know about product updates and latest industry news.